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In vitro effects of WD-3-containing serum on migration capability, and protein and mRNA expression levels in MGC-803 cells. A Cell migration ability detected by scratch assay. B Protein expression of <t>AMPK</t> Thr172 phosphorylation (p-AMPK Thr172) and total PPARγ in MGC-803 cells analyzed by Western blotting. C Western blot analysis of p-AMPK Thr172 and total PPARγ after treatment with an AMPK inhibitor (Compound C <t>2HCl)</t> and a PPARγ inhibitor (T0070907). Phosphorylated PPARγ was not assayed in this study. D mRNA expression of AMPK and PPARγ detected by RT-qPCR. Data are presented as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 vs. Control
Adenosine Monophosphate Activated Protein Kinase Ampk Inhibitor, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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In vitro effects of WD-3-containing serum on migration capability, and protein and mRNA expression levels in MGC-803 cells. A Cell migration ability detected by scratch assay. B Protein expression of AMPK Thr172 phosphorylation (p-AMPK Thr172) and total PPARγ in MGC-803 cells analyzed by Western blotting. C Western blot analysis of p-AMPK Thr172 and total PPARγ after treatment with an AMPK inhibitor (Compound C 2HCl) and a PPARγ inhibitor (T0070907). Phosphorylated PPARγ was not assayed in this study. D mRNA expression of AMPK and PPARγ detected by RT-qPCR. Data are presented as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 vs. Control

Journal: Biological Procedures Online

Article Title: A Clinically Derived TCM Decoction (WD-3) Attenuates Malignant Phenotypes of Gastric Cancer through the PPARγ–AMPK Pathway

doi: 10.1186/s12575-025-00320-2

Figure Lengend Snippet: In vitro effects of WD-3-containing serum on migration capability, and protein and mRNA expression levels in MGC-803 cells. A Cell migration ability detected by scratch assay. B Protein expression of AMPK Thr172 phosphorylation (p-AMPK Thr172) and total PPARγ in MGC-803 cells analyzed by Western blotting. C Western blot analysis of p-AMPK Thr172 and total PPARγ after treatment with an AMPK inhibitor (Compound C 2HCl) and a PPARγ inhibitor (T0070907). Phosphorylated PPARγ was not assayed in this study. D mRNA expression of AMPK and PPARγ detected by RT-qPCR. Data are presented as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 vs. Control

Article Snippet: Adenosine monophosphate-activated protein kinase (AMPK) inhibitor (Compound C 2HCl, S7306, Selleck Chemical, TX, USA) and peroxisome proliferator-activated receptor gamma (PPARγ) inhibitor (S2871, Selleck Chemical, TX, USA) were used in cell induction.

Techniques: In Vitro, Migration, Expressing, Wound Healing Assay, Phospho-proteomics, Western Blot, Quantitative RT-PCR, Control

Effects of WD-3-containing serum on PPARγ-silenced MGC-803 cells post-transfection. MGC-803 cells were transfected with PPARγ siRNA for 48 h, followed by 48 h treatment according to experimental groups. A Cell viability measured by CCK-8 assay. B , C Cell migration capability assessed by scratch assay. D Protein expression of AMPK Thr172 phosphorylation (p-AMPK Thr172) and total PPARγ detected by Western blotting. E mRNA expression of AMPK and PPARγ analyzed by RT-qPCR. Data are presented as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 vs. Control

Journal: Biological Procedures Online

Article Title: A Clinically Derived TCM Decoction (WD-3) Attenuates Malignant Phenotypes of Gastric Cancer through the PPARγ–AMPK Pathway

doi: 10.1186/s12575-025-00320-2

Figure Lengend Snippet: Effects of WD-3-containing serum on PPARγ-silenced MGC-803 cells post-transfection. MGC-803 cells were transfected with PPARγ siRNA for 48 h, followed by 48 h treatment according to experimental groups. A Cell viability measured by CCK-8 assay. B , C Cell migration capability assessed by scratch assay. D Protein expression of AMPK Thr172 phosphorylation (p-AMPK Thr172) and total PPARγ detected by Western blotting. E mRNA expression of AMPK and PPARγ analyzed by RT-qPCR. Data are presented as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 vs. Control

Article Snippet: Adenosine monophosphate-activated protein kinase (AMPK) inhibitor (Compound C 2HCl, S7306, Selleck Chemical, TX, USA) and peroxisome proliferator-activated receptor gamma (PPARγ) inhibitor (S2871, Selleck Chemical, TX, USA) were used in cell induction.

Techniques: Transfection, CCK-8 Assay, Migration, Wound Healing Assay, Expressing, Phospho-proteomics, Western Blot, Quantitative RT-PCR, Control